知母皂甙E1和E2

TIMOSAPONINS E1 AND E2

摘要: 应用硅胶柱色谱、高效液相色谱等分离手段,从中药知母(Anemarrhena asphodeloides Bge.)分离得到2种新的呋甾皂甙,经光谱(IR,ESIMS,¹HNMR,¹³HNMR)分析和化学反应,鉴定其结构分别是:(25S)-26-O-β-D-吡喃葡萄糖基-22-羟基-5-β-呋甾-3β,15α,26-三醇-3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖甙(1),(25S)-26-O-β-D-吡喃葡萄糖基-22-甲氧基-5β-呋甾-3β,15α,26-三醇-3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖甙(2)。(1)是新化合物,命名为知母皂甙E1;(2)是(1)的甲醚化人工产物,命名为知母皂甙E2。对知母皂甙-I(A)的结构分析作了简单说明。

Abstract: By means of silica gel chromatography and HPLC, two compounds were isolated from Anemarrhena asphodeloides Bge.. On the basis of chemical and spectral (MS,¹H,¹³CNMR, IR) analyses, their structures were elucidated as (25S)-26-O-β-D-glucopyranosyl-22-hydroxy-5β-furost-3β,15α,26-triol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (1), and (25S)-26-O-β-D-glucopyranosyl-22-methoxy-5β-furost-3β,15α,26-triol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (2). They are named as timosaponin E1 (1) and timosaponin E2 (2) respectively. The structure of anemarrhenasaponin-I (A) is briefly discussed. Timosaponins are the main active constituents of Anemarrhena asphodeloides, most of them were shown to can inhibit platelet aggregation.