To investigate the effects of lanosterol (1), inotodiol (2) and trametenolic acid (3) from Inonotus obliquus against oxidative damage induced by CCl₄ in mice, 1, 2 and 3 (20, 10 and 5 mg·kg⁻¹) were respectively administered to mice, once a day for 3 days.  Then the mice were induced to oxidative damage by CCl₄ on the third day 30 min after the administration.  The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) and reductive glutathione (GSH) in serum and liver homogenate were determined.  And the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and interleukin-6 (IL-6) concentration in serum were detected.  The results showed that treatment with compound 1, 2 and 3 could significantly increase the activities of SOD, CAT and GSH-PX in serum and liver homogenate.  Furthermore, the content of GSH in serum and liver homogenate increased and MDA content decreased markedly.  In addition, compound 1, 2 and 3 could significantly inhibit the activities of ALT and AST in serum, and decrease the IL-6 concentration in serum remarkably.  So, compound 1, 2 and 3 can protect mice against oxidative stress injury induced by CCl₄.  Furthermore, compound 1, 2 and 3 can protect cells from damage through inhibition on ALT, AST and the expression of IL-6.