杨铁虹, 贾敏, 梅其炳. 当归多糖组分AP-3诱生小鼠脾细胞IL-2和IFN-γ的作用J. 药学学报, 2006, 41(1): 54-57.
引用本文: 杨铁虹, 贾敏, 梅其炳. 当归多糖组分AP-3诱生小鼠脾细胞IL-2和IFN-γ的作用J. 药学学报, 2006, 41(1): 54-57.
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YANG Tie-hong, JIA Min, MEI Qi-bing. Effect of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-γ inductionJ. Acta Pharmaceutica Sinica, 2006, 41(1): 54-57.
Citation: YANG Tie-hong, JIA Min, MEI Qi-bing. Effect of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-γ inductionJ. Acta Pharmaceutica Sinica, 2006, 41(1): 54-57.
shu

当归多糖组分AP-3诱生小鼠脾细胞IL-2和IFN-γ的作用

Effect of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-γ induction

    摘要
  • 摘要: 目的研究当归多糖组分AP-3对细胞因子IL-2和IFN-γ的诱生作用，以探讨其免疫调节的特点。方法 流式细胞术测定培养的脾细胞中CD4+细胞比例；酶联免疫法测定培养上清液中IL-2和IFN-γ的浓度；RT-PCR法测定IL-2和IFN-γ mRNA的转录水平。结果AP-3在0.6～2 μmol·L-1，能显著提高培养脾细胞CD4+细胞的百分率；在2～6 μmol·L-1，AP-3时间、剂量依赖性地增加培养的细胞上清液中IL-2的浓度和细胞内IL-2 mRNA的转录水平，而对于IFN-γ和IFN-γ mRNA，则先升高后降低，并呈现剂量依赖性。结论当归多糖能够促进IL-2和IFN-γ的分泌，激活Th1细胞，从而发挥免疫调节作用。

     

    Abstract: AimTo investigate the effects of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-γ induction and its further immunomodulatory feature. MethodsThe percentage of CD4+ lymphocyte was detected by flow cytometric method, the production of IL-2 and IFN-γ in cell culture supernatant were determined by ELISA, mRNA expressions of IL-2 and IFN-γ cytokines were detected by RT-PCR. ResultsAt the range of 0.6-2 μmol·L-1, AP-3 significantly enhanced the percentage of CD4+ lymphocytes in total splenocytes. At the range of 2-6 μmol·L-1, the treatment of AP-3 augmented both productions of IL-2 in cell culture supernatant and cell IL-2 mRNA transcription level in a time and dose dependent manner. While in the case of IFN-γ, AP-3 stimulated at early time after exposure but down-regulated thereafter. ConclusionAngelica sinensis polysaccharide could regulate the immune response through upregulating IL-2, IFN-γ expression and activating Th1 cell.

     

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