GABAA受体对鞘内注射氧化槐定碱镇痛作用的影响
The influence of GABAA receptor on the analgesic action of intrathecally injected oxysophoridine
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摘要:
观察氧化槐定碱 (oxysophoridine, OSR) 鞘内注射 (intrathecal injection, ith) 的镇痛作用并探讨与GABAA受体相关的作用机制。采用小鼠温浴法测定痛阈, 观察OSR (ith) 的镇痛作用及GABAA受体激动剂和拮抗剂对OSR镇痛作用的影响; 采用免疫组化法检测OSR对小鼠脊髓背角GABAARα1蛋白表达的影响。结果显示, OSR (12.5和6.25 mg·kg−1, ith) 能明显延长热甩尾潜伏期 (P < 0.05, P < 0.01), 痛阈提高率达68.45%。GABA和蝇蕈醇 (MUS) 与OSR有协同镇痛作用, 印防己毒素 (PTX) 和荷包牡丹碱 (BIC) 能拮抗OSR的镇痛作用。OSR (12.5 mg·kg−1, ith) 可使脊髓背角GABAARα1表达明显增多 (P < 0.01)。结果提示, OSR (ith) 具有明显的镇痛作用, 脊髓GABAA受体参与了OSR的镇痛机制。
Abstract:This study is to investigate the analgesic effect produced by intrathecal injection (ith) of oxysophoridine (OSR) and the mechanism of GABAA receptor. Warm water tail-flick test was used to detect the analgesic effect of OSR (12.5, 6.25, and 3.13 mg·kg−1, ith) and to observe the influence of GABA (gamma aminobutyric acid) agonist or antagonist on the analgesic effect of OSR in mice. Immunohistochemistry method were used to detect the influence of OSR (12.5 mg·kg−1, ith) on the GABAARα1 protein expression in spinal cord. The results obtained covers that OSR (12.5 and 6.25 mg·kg−1, ith) alleviates pain significantly with the warm water tail-flick test (P < 0.05, P < 0.01), the rate of pain threshold increases by 68.45%; GABA and muscimol (MUS) produces analgesic synergism together with the OSR, picrotoxin (PTX) and bicuculline (BIC) antagonize the analgesic effect of OSR; OSR (12.5 mg·kg−1, ith) significantly increase the positive number of GABAARα1 nerve cell in spinal cord (P < 0.01) and significantly decrease the average grey levels (P < 0.01). In conclusion, OSR intrathecal injection has significant analgesic effect. And GABAA receptor in spinal cord is involved in the analgesic mechanism.
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