苦参与苦豆子中生物碱的高效液相层析法与薄层光密度法测定

HPLC AND TLC-DENSITOMETRY DETERMINATION OF ALKALOIDAL CONSTITUENTS IN SOPHORA FLAVESCENS AND SOPHORA LOPECUROIDES

摘要: 本文报道苦参与苦豆子中五种生物碱的两种微量测定方法——高效液相层析法与薄层光密度法。前法重现性好,纯品的回收率为98.2～99.3%,后法操作简便不需净化,线性范围为1～4μg。方法的变异系数分别为0.68～1.74%及1.62～2.77%,两法测定结果一致。

Abstract: This paper deals with two analytical methods (HPLC and TLCdensitometry) of alkaloids in Sophora flavescens Ait. and Sophora alopecuroides Linn. Sophocarpine (Ⅰ), matrine (Ⅱ), sophoridine (Ⅲ), oxymatrine (Ⅳ) and oxysophocarpine (Ⅴ) all were fairly separated either on alkaline silica gel G thin layer by step-wise development or on HPLC silica gel column with two mobile phases. Cinchonidine and atropine were chosen as internal standard respectively and quantified by peak height ratio in the HPLC method.Both methods are sensitive, simple and rapid. The CV% are 1.62～2.77% and 0.6～1.74% respectively. Analytical results obtained by these two methods are in good agreement.