Abstract: AIMTo study the chemical constituents from Uvaria macrophylla Roxb. (Annonaceae). METHODSVarious chromatography techniques were used to separate and purify the constituents. Their structures were elucidated by UV, IR, MS, ¹HNMR, ¹³CNMR, ¹H-¹H COSY, HMQC and HMBC spectral analysis. RESULTS Seven compounds have been isolated from the CHCl₃ extract of the roots of the U.macrophylla. They were identified as macrophyllin (1), onysilin (2), taraxerol (3), 3,5-dimethoxy benzyl benzoic acid ester (4), benzoic acid (5), β-sitosterol (6) and daucosterol (7). CONCLUSIONCompound 1 is a new compound. Compounds 2～7 were obtained from this plant for the first time.