Abstract: AimTo determine 3 kinds of biologically active components (9 compounds) in Gardenia, namely geniposidic acid, gentiobioside, gardenoside, genipin geniposide, chlorogenic acid, crocin 1, crocin 2, crocin 3 and crocetin simultaneously and quantitively. MethodsAn HPLC method detected by 3 different UV-vis waves — 240 nm for geniposidic acid, geniposide, gardenoside, genipin gentiobioside,330 nm for chlorogenic acid, 440 nm for crocin 1, crocin 2, crocin 3 and crocetin respectively, has been developed: Phenomenex Luna C₁₈ column（250 mm×4.6 mm ID，5 μm）with linear gradient elution using methanol-acetotrile(9∶1) and 0.3% aqueous formic acid. ResultsThis method successfully determined 9 compounds in Gardenia (from 5 different areas) simultaneously and quantitively. ConclusionThis is a more reasonable and credible quality control method for the Chinese traditional medicine.