异地衣糖和藏红花醇提物对海马齿状回长时程增强影响的比较研究
Comparison of the action of isolichenin and methanol extract of saffron on long-term potentiation in hippocampal dentate gyrus in vivo
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摘要:
长时程增强是神经系统信息储存的细胞水平模型, 被认为是学习和记忆的形成机制。长时程增强电生理技术常用于促进记忆药物的筛选实验。海马齿状回长时程增强筛选研究发现, 异地衣糖能在20 pulses/100 Hz的刺激条件下诱导出长时程增强, 而单独应用该刺激条件并不能诱导出长时程增强。研究经验表明具有这样作用的受试药物, 往往能够拮抗30%乙醇抑制30 pulses/60 Hz刺激条件诱导的长时程增强, 比如藏红花醇提物。但在随后的研究中发现, 异地衣糖不能拮抗30%乙醇对30 pulses/60 Hz刺激条件诱导的长时程增强, 提示异地衣糖与藏红花醇提物的促进长时程增强作用机制不同, 是通过某一未知的新机制实现的。
Abstract:Long-term potentiation (LTP) is thought as a generative mechanism underlying learning and memory via storing information in central nervous system. Electro-neurophysiological assay for LTP is generally used in screening the drugs that can facilitate learning and memory. By using in vivo LTP technique, isolichenin was found to facilitate LTP induction by a tetanic stimulation (20 pulses/100 Hz) in dentate gyrus. This tetanic stimulation by itself, however, cannot induce LTP. Previous study showed the reagent being able to facilitate LTP-induction, like methanol extract of saffron (MES), usually can antagonize the inhibiting effect of 30% ethanol on LTP induction (30 pulses/60 Hz). Isolichenin may also fall into such kind of drugs. Interestingly, comparatively study showed that isolichenin failed to antagonize the inhibiting effect of 30% ethanol on LTP induction (30 pulses/60 Hz). This result indicates a different unknown mechanism existing in the effect of isolichenin on LTP or memory formation.
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