褪黑素对大脑皮层细胞一氧化氮含量及其神经毒性作用的影响

EFFECTS OF MELATONIN ON NITRIC OXIDE CONTENT AND ITS NEUROTOXICITY IN CEREBRAL CORTEX

摘要: 目的：探讨褪黑素(MT)抗衰老作用与神经细胞NO释放之间的联系。方法：连续给予老年小鼠MT，检测大脑皮层神经细胞NO含量的变化,并用原代培养的大鼠皮层神经元，去血清培养后，观察MT对高钾、谷氨酸（Glu）诱发NO释放及硝普钠（SNP）致神经毒性作用的影响。结果：MT能明显抑制老年小鼠脑内NO含量的增高，并拮抗KCI与Glu诱发的NO释放及SNP引起的神经毒性，对大脑神经元有保护作用。结论：MT抑制大脑皮层NO含量增高，可能是其抗衰老作用的机制之一。

Abstract: AIM: To explore the mechanisms of the antiaging action of melatonin(MT) . METHODS: The effects of MT on nitric oxide (NO) content, high concentrations of KCI, glutamate (Glu)-induced NO release, and sodium nitroprusside (SNP)-elicited neurotoxicity were studied in old mouse cerebral cortex and rat primary cortical cultures. RESULTS: MT could inhibit the elevation of NO content in old mouse cerebral cortex, reduce the KCI, Glu-induced NO release, antagonize the SNP-induced neurotoxicity in cultured cortical cells of fetal rat. CONCLUSION: The inhibitory effects on NO release and its neurotoxicity were probably one of the mechanisms of the MT antiaging action.