褶合光谱法用于DNA样品中杂质的限量检查

DETECTION OF IMPURITIES IN DNA SAMPLES BY USING THE CONVOLUTION SPECTRUM METHOD

摘要: 目的　对DNA样品中苯酚、氨基酸和内毒素进行限量检测。方法　采用褶合变换技术、光谱空间理论提出的相关系数判别分析法和计算机信息处理技术进行杂质检查。结果　褶合光谱法与二阶导数光谱法及A₂₆₀/A₂₈₀比值法结果相对照。表明本法可对DNA样品中杂质进行半定量检测。结论　本法快速、简便、准确，可用于生化样品分析。

Abstract: AIM　To detect the presence of phenol, amino acids and endotoxin in DNA preparations. METHODS　By using the convolution curve transformation technology, correlation coefficient discriminant which was put forward by spectrum space theory and computer information process technology, impurities in DNA samples were detected. RESULTS　The convolution spectrum method compared with A₂₆₀/A₂₈₀ ratio method and second-derivative absorption spectrum method could be used for the semiquantitative determinations of impurities in DNA samples and the results were satisfactory. CONCLUSION　The method was simple, convenient, fast and valuable to the biochemical analysis.