丹参毛状根中多种丹酚酸类化合物的鉴定与生物合成(英文)

赵淑娟  张金家  杨  莉  王峥涛  胡之璧

丹参毛状根中多种丹酚酸类化合物的鉴定与生物合成(英文)

赵淑娟张金家杨莉王峥涛胡之璧

Determination and biosynthesis of multiple salvianolic acids in hairy roots of Salvia miltiorrhiza

DIAO Chu-Juan

摘要

摘要:
应用发根农杆菌9402侵染丹参叶片获得了一株可以稳定产生多种丹酚酸类化合物的丹参毛状根。除迷迭香酸和丹酚酸B外还含有丹酚酸K、丹酚酸L、乙基丹酚酸B、甲基丹酚酸B以及一个分子量为538的未知化合物 (化合物538)。LC-MS对这些化合物进行了鉴定。对茉莉酸甲酯 (MeJA) 和酵母诱导子对几种主要化合物积累的影响进行了分析: MeJA促进丹酚酸B、迷迭香酸、丹酚酸K和化合物538的积累, 分别从干重4.21%、2.48%、0.29% 和0.01% 提高到7.11%、3.38%、0.68% 和0.04%; 酵母诱导子提高迷迭香酸含量, 从干重2.83%上升到5.71%, 抑制了丹酚酸B、丹酚酸K和化合物538的生物合成。实验结果表明所获得的丹参毛状根可以作为生产丹酚酸B、迷迭香和丹酚酸K以及化合物538的替代资源。对这些酚酸类化合物对诱导子的生物累积效应变化趋势进行分析推测：丹参毛状根中丹酚酸K和化合物538可能是从迷迭香酸合成丹酚酸B的中间产物。

Abstract:
Danshen (Salvia miltiorrhiza Bunge) hairy roots were obtained by infecting Danshen leaves with Agrobacterium rhizogenes 9402. Besides rosmarinic acid (RA) and salvianolic acid B (SAB), the hairy root could also produce salvianolic acid K (SAK), salvianolic acid L, ethyl salvianolic acid B (ESAB), methyl salvianolic acid B (MSAB), and a compound with a molecular weight of 538 (compound 538) identified by using LC-MS. Effects of methyl jasmonate (MeJA) and yeast elicitor (YE) on the accumulation of these compounds had been investigated. MeJA increased the accumulation of SAB, RA, SAK, and compound 538 from 4.21%, 2.48%, 0.29%, and 0.01% of dry weight to 7.11%, 3.38%, 0.68%, and 0.04%, respectively. YE stimulated the biosynthesis of RA from 2.83% to 5.71%, but depressed the synthesis of SAB, SAK and compound 538. It was indicated in all the results that these Danshen hairy roots could be used as alternative resources to produce salvianolic acids. Analysis of the content variation of these compounds after elicitation suggested that SAK and compound 538 might be the intermediates in the biosynthesis from RA to SAB in Danshen hairy roots.

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