金银花类药用植物FatB基因克隆和生物信息学分析
Cloning and bioinformatic analysis of FatB genes in Lonicera japonica Thunb and its substitutes
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摘要:
从忍冬 (Lonicera japonica Thunb.) 转录组测序结果中分析获得1个FatB基因。分别以忍冬、红白忍冬 (Lonicera japonica Thunb. var. chinensis (Wats.) Bak.)、红腺忍冬 (Lonicera hypoglauca Miq.) 和水忍冬(Lonicera dasystyla Rehd.) 新鲜花蕾为材料, 利用RT-PCR技术克隆获得了LJFatB、LHFatB、LJCFatB、LDFatB基因的全长cDNA, 并进行生物信息学分析。结果表明LJFatB、LJCFatB、LHFatB和LDFatB与拟南芥AtFatB具有较近的亲缘关系。LJFatB、LJCFatB、LHFatB和LDFatB的核苷酸序列、蛋白特性及其二级结构有所差异, 但其蛋白均具有保守的FatB底物结合位点和催化活性位点, 且在花蕾中的转录水平没有显著差异。因此推测LJFatB、LJCFatB、LHFatB和LDFatB可能具有与AtFatB相同的生物学功能。
Abstract:A FatB unigene was obtained from the transcriptome dataset of Lonicera japonica Thunb. Full-length FatB cDNA was cloned from buds of Lonicera japonica Thunb., Lonicera japonica Thunb. var. chinensis (Wats.) Bak., Lonicera hypoglauca Miq. and Lonicera dasystyla Rehd. using RT-PCR technology, and named as LJFatB, LHFatB, LJCFatB and LDFatB. The results of bioinformatic analysis showed that LJFatB, LJCFatB, LHFatB and LDFatB and Arabidopsis thaliana AtFatB had a closely relationship. Nucleotide sequences and protein secondary structure of LJFatB, LJCFatB, LHFatB and LDFatB are different and their proteins had conserved FatB substrate binding sites and catalytic activity sites. Transcriptive level of LJFatB, LJCFatB, LHFatB and LDFatB in bud was not significantly different. Therefore, LJFatB, LJCFatB, LHFatB and LDFatB could have the same biological function as AtFatB.
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