Being an essential component of systematic biology, metabolomics has received attention in   recent years.  It is a post genomic technology aimed at qualitative and quantitative analysis of all low molecular- mass metabolites present in complex biological samples, and mainly investigates the change of endogenous  metabolites of a stimulated or disturbed biological system.  Investigations into intracellular endogenous     metabolites in metabolomics have great advancement in recent years.  This review outlines the progress of    metabolomics in cell culture analysis including sample preprocessing methods and metabolite target analysis, metabolic profiling analysis, metabolomics analysis and metabolic footprinting analysis.