臧宝霞, 金鸣, 吴伟, 陈文梅, 朴永哲, 李金荣. 杨梅素对血小板活化因子拮抗的作用J. 药学学报, 2003, 38(11): 831-833.
引用本文: 臧宝霞, 金鸣, 吴伟, 陈文梅, 朴永哲, 李金荣. 杨梅素对血小板活化因子拮抗的作用J. 药学学报, 2003, 38(11): 831-833.
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ZANG Bao-xia, JIN Ming, WU Wei, CHEN Wen-mei, PIAO Yong-zhe, LI Jin-rong. Antagonistic effect of myricetin on platelet activing factorJ. Acta Pharmaceutica Sinica, 2003, 38(11): 831-833.
Citation: ZANG Bao-xia, JIN Ming, WU Wei, CHEN Wen-mei, PIAO Yong-zhe, LI Jin-rong. Antagonistic effect of myricetin on platelet activing factorJ. Acta Pharmaceutica Sinica, 2003, 38(11): 831-833.
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杨梅素对血小板活化因子拮抗的作用

Antagonistic effect of myricetin on platelet activing factor

    摘要
  • 摘要: 目的研究杨梅素对血小板活化因子（PAF）的拮抗作用。方法以放射配体结合试验观察［3H］PAF与家兔血小板受体特异性结合的作用；以分光光度法测定PAF诱发血小板粘附的强度；以Fura-2荧光分光光度法测定兔多形核白细胞（PMNs）内钙离子浓度。结果杨梅素可浓度依赖地抑制［3H］PAF与血小板受体的特异性结合，其IC50分别为 34.8，85.7和118.6 μmol·L-1；该药可明显抑制PAF诱发的血小板粘附及PMNs内游离钙浓度的升高，且呈明显的量效关系，其抑制血小板粘附的IC50为 13.1 μmol·L-1。结论杨梅素具有抗PAF作用，为一新的PAF受体拮抗剂。

     

    Abstract: AimTo study the antagonistic effect of myricetin on platelet activing factor (PAF). MethodsThe specific binding of ［3H］ PAF to rabbit platelet receptor was investigated using radio ligand binding assay (RLBA). Platelet adhesion induced by PAF was measured with spectrophotometry. The elevation of inner free calcium concentration in rabbit polymorphonuclear leukocytes (PMNs) induced by PAF was assayed by Fura-2 fluorescent technique. ResultsThe specific binding inhibition potency of Myr was found to be concentration-dependent. The IC50 of Myr in ［3H］　PAF 1, 2 and 4 nmol·L-1 were 34.8, 85.7 and 118.6 μmol·L-1, respectively. The PAF induced reactions of rabbit platelet adhesion and PMNs inner free calcium concentration increase were inhibited by Myr in a dose-dependent manner. The IC50 of Myr to inhibit platelet adhesion was 13.1 μmol·L-1. ConclusionThe specific receptor binding of PAF can be antagonized by myricetin.

     

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