李泽松, 李德良, 黄坚, 丁雨, 马百平, 王升启. 心血管相关基因芯片的制备及其在知母皂苷作用机理研究中的应用心血管相关基因芯片的制备及其在知母皂苷作用机理研究中的应用J. 药学学报, 2003, 38(7): 496-500.
引用本文: 李泽松, 李德良, 黄坚, 丁雨, 马百平, 王升启. 心血管相关基因芯片的制备及其在知母皂苷作用机理研究中的应用心血管相关基因芯片的制备及其在知母皂苷作用机理研究中的应用J. 药学学报, 2003, 38(7): 496-500.
shu
LI Ze-song, LI De-liang, HUANG Jian, DING Yu, MA Bai-ping, WANG Sheng-qi. Investigations on the molecular mechanisms of saponins from Anemarrhena asphodeloides Bunge using oligonucleotide microarraysJ. Acta Pharmaceutica Sinica, 2003, 38(7): 496-500.
Citation: LI Ze-song, LI De-liang, HUANG Jian, DING Yu, MA Bai-ping, WANG Sheng-qi. Investigations on the molecular mechanisms of saponins from Anemarrhena asphodeloides Bunge using oligonucleotide microarraysJ. Acta Pharmaceutica Sinica, 2003, 38(7): 496-500.
shu

心血管相关基因芯片的制备及其在知母皂苷作用机理研究中的应用心血管相关基因芯片的制备及其在知母皂苷作用机理研究中的应用

Investigations on the molecular mechanisms of saponins from Anemarrhena asphodeloides Bunge using oligonucleotide microarrays

    摘要
  • 摘要: 目的研究知母皂苷活血化瘀作用的分子机制。方法制备了包含87个心血管疾病相关基因探针的寡核苷酸芯片。将80 mg·L-1 知母皂苷作用8 h及未作用的培养人血管内皮细胞提取总RNA，通过逆转录分别标记Cy 5和Cy 3荧光染料，然后与芯片杂交，检测知母皂苷对血管内皮细胞中心血管疾病相关基因表达的影响。结果知母皂苷作用于血管内皮细胞后，血管紧张素酶原（AGT）基因、肾上腺素α2A受体（ADRA2R）基因和内皮素转换酶-1（ECE-1）基因的表达均有不同程度的下调。结论知母皂苷有调控血管内皮细胞功能的作用，可能是其治疗心血管疾病的机制之一。

     

    Abstract: AimTo investigate the molecular mechanisms of saponins from the rhizome of Anemarrhena asphodeloides Bunge. MethodsOligonucleotide microarrays consisting of 87 probes representing 87 human cardiovascular disease-related genes were constructed. Effects of saponins on gene expression in human umbilical vein endothelial cells were analyzed by comparing hybridization of Cy 5-labeled cDNAs from saponins-treated human umbilical vein endothelial cells and Cy 3-labeled cDNAs from untreated human umbilical vein endothelial cells. ResultsThe results indicate that angiotensinogen gene, α2A-adrenoceptor gene and endothelin-converting enzyme 1 gene were downregulated 2.8, 1.9 and 3.1 folds respectively after human umbilical vein endothelial cells were incubated in medium containing 80 mg·L-1 saponins. ConclusionThese results suggest that saponins may have beneficial effect on cardiovascular diseases by modulating the function of vein endothial cells and microarray can be used to investigate the biological action of extracts from traditional Chinese medicine.

     

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