丹参与三七配伍对主要活性成分药代动力学行为的影响

Effects of combination of Salvia miltiorrhiza and Panax notoginseng on the pharmacokinetics of their major bioactive components in Beagle dog

摘要:
比格犬分别灌胃给药丹参、三七以及丹参-三七配伍的混悬液, 用LC-MS/MS法测定犬血浆中丹参素、丹参酮Ⅱ_A、隐丹参酮、三七皂苷R₁、人参皂苷Rg₁和人参皂苷Rb₁的浓度, 应用BAPP2.0计算药代动力学参数并进行统计分析, 通过比较药代动力学参数在配伍前后的变化探讨丹参与三七的配伍对这6个成分药代动力学行为的影响。结果显示, 配伍后丹参素、三七皂苷R₁、人参皂苷Rg₁和Rb₁的C_max均减少且AUC明显降低、CL_z/F和V_z/F均有一定程度的增加, 而丹参酮Ⅱ_A、隐丹参酮的药代动力学行为未见明显变化。

Abstract:
After oral administration of Salvia miltiorrhiza (Danshen in Chinese), Panax notoginseng (Sanqi in Chinese) and Danshen Sanqi combination suspensions to Beagle dogs, the plasma concentration-time profiles of danshensu, tanshinone Ⅱ_A, cryptotanshinone, notoginsenoside R1, ginsenoside Rg1 and Rb1 were analyzed by LC-MS/MS. Pharmacokinetic parameters were calculated and analyzed with BAPP 2.0 software. The results showed that the C_max and AUC of danshensu, notoginsenoside R1, ginsenoside Rg1 and Rb1 in Danshen Sanqi combination group all decreased in comparison with those of Danshen or Sanqi given alone, while the CL_z/F and V_z/F increased to some extent. No significant differences of the pharmacokinetics of tanshinone Ⅱ_A and cryptotanshinone were observed between groups.