张黎, 芮耀诚, 邱彦, 李铁军, 刘厚佳, 陈万生. 何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响J. 药学学报, 2004, 39(6): 406-409.
引用本文: 张黎, 芮耀诚, 邱彦, 李铁军, 刘厚佳, 陈万生. 何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响J. 药学学报, 2004, 39(6): 406-409.
shu
ZHANG Li, RUI Yao-cheng, QIU Yan, LI Tie-jun, LIU Hou-jia, CHEN Wan-sheng. Expression of VEGF in endothelial cells and the effects of2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucosideJ. Acta Pharmaceutica Sinica, 2004, 39(6): 406-409.
Citation: ZHANG Li, RUI Yao-cheng, QIU Yan, LI Tie-jun, LIU Hou-jia, CHEN Wan-sheng. Expression of VEGF in endothelial cells and the effects of2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucosideJ. Acta Pharmaceutica Sinica, 2004, 39(6): 406-409.
shu

何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对内皮细胞表达VEGF的影响

Expression of VEGF in endothelial cells and the effects of2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside

    摘要
  • 摘要: 目的研究何首乌水溶性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡糖苷(ST I)对溶血磷脂酰胆碱(LPC)诱导人脐静脉内皮细胞株ECV304细胞中血管内皮生长因子(VEGF)表达的影响。方法在ECV304细胞培养基中加入LPC(2.5 mg·L-1)或LPC与ST I共孵24 h，收集各组条件培养基，用基础酶联免疫吸附试验(ELISA)检测各组条件培养基中VEGF蛋白含量；用原位杂交法、RT-PCR及Realtime RT-PCR法检测LPC对内皮细胞VEGF mRNA的表达及ST I的影响。结果ECV304细胞暴露于LPC后，VEGF蛋白分泌明显增加；加入ST I后VEGF蛋白含量明显降低; LPC可以使ECV304中VEGF mRNA的表达明显升高, 并使VEGF165 mRNA的表达升高；ST I可剂量依赖性地抑制VEGF165 mRNA的高表达。结论LPC能诱导ECV304细胞表达高水平的VEGF蛋白及VEGF mRNA，1×10-5 mol·L-1 ST I可抑制LPC的作用，降低VEGF的高表达。

     

    Abstract: AimTo determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304) and the inhibitory effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (ST I) in vitro. Methods Exposure to 2.5 mg·L-1 LPC or LPC+ST I for 24 hours, VEGF protein was determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, VEGF mRNA expression in ECV304 was examined by in situ hybridization. VEGF165 mRNA was examined by RT-PCR and Realtime RT-PCR. ResultsLPC upregulated VEGF protein and VEGF mRNA expression in the ECV304 cells. ST I was shown to markedly inhibit the LPC-induced increase of VEGF protein and VEGF165 mRNA (P<0.001). ConclusionLPC can induce a strong expression of VEGF in ECV304 cells and ST I can inhibit it.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回