黄绵庆，杨照新，姚茂忠，田树红，符 健. 葛根素通过神经酰胺通路抑制UVB诱导的HaCaT细胞DNA损伤J. 药学学报, 2012,47(5): 609-613.
引用本文: 黄绵庆，杨照新，姚茂忠，田树红，符 健. 葛根素通过神经酰胺通路抑制UVB诱导的HaCaT细胞DNA损伤J. 药学学报, 2012,47(5): 609-613.
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HUANG Mian-qing, YANG Zhao-xin, YAO Mao-zhong, TIAN Shu-hong, FU Jian. Puerarin inhibits DNA damage of HaCaT cells induced by UVB via ceramide pathwayJ. 药学学报, 2012,47(5): 609-613.
Citation: HUANG Mian-qing, YANG Zhao-xin, YAO Mao-zhong, TIAN Shu-hong, FU Jian. Puerarin inhibits DNA damage of HaCaT cells induced by UVB via ceramide pathwayJ. 药学学报, 2012,47(5): 609-613.
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葛根素通过神经酰胺通路抑制UVB诱导的HaCaT细胞DNA损伤

Puerarin inhibits DNA damage of HaCaT cells induced by UVB via ceramide pathway

    摘要
  • 摘要:

    研究葛根素 (puerarin, PUE) 对UVB诱导的HaCaT细胞DNA损伤的影响和作用机制。葛根素预处理细胞以30 mJ·cm−2 UVB照射, 24 h后以彗星电泳实验检测DNA损伤, 薄层色谱法和气相色谱法检测神经酰胺, 流式细胞术检测细胞内游离钙离子, Western blotting法检测p38蛋白磷酸化水平。结果显示, UVB模型组细胞DNA损伤、细胞内游离钙离子、神经酰胺、磷酸化p38蛋白显著增加; 与UVB模型组相比, 各葛根素预处理组的DNA损伤、细胞内游离钙离子、神经酰胺、磷酸化p38蛋白均减少。结果表明, 葛根素首先抑制神经酰胺蓄积, 阻止下游的细胞内钙离子升高和p38 MAPK通路激活, 从而减轻UVB诱导的HaCaT细胞DNA损伤。

     

    Abstract:

    This study is to investigate the effect and mechanism of puerarin on DNA damage of HaCaT cells induced by UVB.  Puerarin pre-treated cells were irradiated with UVB at 30 mJ·cm−2.  Twenty four hours after irradiation, DNA damage was detected by comet assay, ceramide was measured by thin layer chromatography and gas chromatography, intracellular free calcium ion was analyzed by flow cytometry, the phosphorylation level of p38 protein was examined by Western blotting method.  Levels of DNA damage, ceramide, free calcium ion and p-p38 protein were elevated in UVB model cells.  Contrary to the model group, all indicators above were reduced in all groups pre-treated by puerarin.  Puerarin restrains the ceramide accumulation to block downstream p38 MAPK pathway and calcium ion rising, therefore reduces DNA damage in HaCaT cells induced by UVB.

     

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