Abstract: AimTo study mRNA expression alteration of two-pore potassium channels in the brain of β-amyloid peptide_25-35(β-AP_25-35)-induced memory impaired rats.MethodsMemory impairments induced in rats by single icv injection of β-AP_25-35 (2 mmol·L^-1) 5 μL were assessed in the Morris water maze test.The mRNA expression levels of three two-pore potassium channels TREK-1, TREK-2 and TRAAK were detected in rat cerebral cortex and hippocampus by reverse transcription-polymerase chain reaction (RT-PCR).ResultsIn the Morris water maze test, the escape latencies of the β-AP_25-35-treated rats were longer than those of the control group in 1st, 2nd and 4th　training day, suggesting that the memory of β-AP_25-35-treated rats was obviously impaired.Compared with the control group, the mRNA levels of TREK-1, TREK-2 and TRAAK in the hippocampus of the β-AP_25-35-treated rats were increased by 40.0%, 27.9% and 18.9%, respectively; while no significant change of TREK-1, TREK-2 and TRAAK mRNA levels was observed in the cortex.Conclusion The mRNA expression levels of two-pore potassium channels were increased significantly in the brain of β-AP_25-35-induced memory impaired rats.