Abstract: AimTo study the chemical constituents of Dryopteris sublaeta Ching et Hsu.Methods Fresh plant of Dryopteris sublaeta Ching et Hsu was extracted twice with boiling water, the extract was concentrated to small volume under reduced pressure at 50 ℃.The concentrated material was partitioned with ether, ethyl acetate, and n-butanol.The fraction of ether extract was chromatographed over silica gel column.The compounds were identified on the basis of their physiochemical and spectral data.Results Four compounds were obtained and identified as 2(S)-5,7,3′-trihydroxy-6,8-dimethyl-5′-methoxy-flavanone (1), matteucinol (2), desmethoxymatteucinol (3) and 5,7,2′-trihydroxy-6,8-dimethyl-flavanone (4).ConclusionCompound 1 is a new one, the others were isolated from Dryopteris for the first time.