Abstract: AIM: To study the protective effect of ebselen on liver damage. METHODS: ICR mice and cultured hepatocytes were both treated with CCl₄ and lipopolysaccharide+D-galactosamine. Pathological changes and glutamine-pyruvic transaminase(ALT), total bile acid（TBA）as well as total bilirubin（TBIL）contents in mouse serum were determined. RESULTS: The LDH activity in supernatant and thiobarbituric acid reactive substance(TBARS) content of cultured hepatocytes were evaluated. Results showed that ebselen ameliorated the pathological changes of the mouse liver and decreased the contents of ALT, TBA and TBIL. The LDH efflux and TBARS increase in hepatocytes were also depressed by ebselen. CONCLUSION: The above results indicate that ebselen is effective against liver damage, which may derive from its anti-lipid oxidation capacity.