Abstract: A method was developed for the determination of oxiracetam concentration in serum and urine by HPLC. Acyclovir was used as an internal standard.The analytical column was a stainless-steel column(3 0 mm×4.6 mm ID ) filled with 10 μm Bondapak NH2.A mixture of acetonitrile and water(80 :20)as mobile phase was used at a flow rate of l ml·min^-1.Detection was performed at 210 nm. The retention times were 6.3 min for oxiracetam and 8.1 min for the internal standard.The lower detection limits were 1μg· mL^-1 for serum and 20μg· mL^-1 for urine. The precision and accuracy within-day and day-to-day for both serum and urine samples ranged from 5.0 to 10.7%. The mean recoveries were 99.7±5.9% and 99.0±5.6% for human serum and urine ,respectively.The results showed that the method is simple,rapid ,sensitive ,reliable and good enough to be used in studying the clinical parmacokinetics of oxiracetam.