Abstract: AimTo prepare acyclovir liposome for improvement the entrapment efficiency and stability.MethodsAcyclovir liposome was prepared by the reverse evaporating method. Surfactants such as sodium deoxycholate and oleic acid were added to optimize the conditions and technology of preparing acyclovir liposome. The entrapment efficiency and particle size of the acyclovir liposome were determined. The liposome stability was proved by centrifugal acceleration experiment. ResultsThe particle size of the acyclovir liposome was 219.8 nm with the polydispersity index of 0.158. The entrapment efficiency reached 65%. The liposome was stable. ConclusionThe results suggest that the conditions and technology are stable and practical to prepare the liposome with high entrapment efficient and stability.