竹黄多糖SB-Ⅰ及SB-Ⅱ的组成研究——Ⅰ.竹黄多糖SB-Ⅰ及SB-Ⅱ的检定及克分子比测定

STUDIES ON THE CONSTITUENTS OF POLYSACCHARIDES SB-Ⅰ AND SB-Ⅱ FROM SHIRAIA BAMBUSICOLA——Ⅰ. IDENTIFICATION OF POLYSACCHARIDES SB-Ⅰ AND SB-Ⅱ FROM SHIRAIA BAMBUSICOLA AND DETERMINATION OF THEIR MOLAR RATIOS

摘要: 将水解后的多糖转化成硅醚衍生物和还原乙酰化衍生物,用气相层析法和气相-质谱法检定了竹黄多糖SB-Ⅰ由L-阿拉伯糖、D-半乳糖、D-葡萄糖组成,竹黄多糖Sb-Ⅱ由L-阿拉伯糖、D-甘露糖、D-半乳糖、D-葡萄糖组成。纸层析未检出阿拉伯糖。采用OV-225固定液的填充柱,对L-阿拉伯糖、D-核糖、D-木糖、L-鼠李糖、D-甘露糖、D-半乳糖、D-葡萄糖七种单糖的还原乙酰化衍生物的分离检定获得满意结果。并在该柱上测定了竹黄多糖组成中的单糖的克分子比。

Abstract: After conversion of the hydrolyzcd polysaccharidcs SB-Ⅰ, SB-Ⅱ from Shiraia Bambusicola into trimethylsilyl and alditol acetate derivatives the components of these polysaccharidcs were identified by GC and GC-MS. SB-Ⅰ is composed of L-arabinose, D-galactosc and D-glucose. SB-Ⅱ is composed of the same three sugars and an additional D-mannose. The molar ratios of these sugars were also determined.The column, packed with 5% OV-225 on Aw-DMCS-Chromosorb W(80/100 mesh), is used satisfactorily for separating and identifying alditol acetate derivatives of the seven sugars (L-arabinose, D-galactose, D-glucose, D-mannose, D-ribose, L-rhamnose).