天然产物液相色谱-质谱-数据库 (LC-MS-DS) 的建立与应用

Construction and application of natural products LC-MS-DS

摘要:
为克服当前天然产物研究方法存在的缺陷, 本文利用高效液相-电喷雾离子阱串联质谱 (HPLC-ESI- IT-MS/MS) 建立了含有600多个化合物的液相色谱-质谱-数据库 (LC-MS-DS), 并对其谱库检索结果的可信度进行了验证。结果表明, LC-MS-DS的检索结果具有很高的可信度, 可以应用于天然产物未知组分的高效鉴定和靶向分离。本研究为天然产物的快速识别与高效分离提供了有效的方法。

Abstract:
To overcome the defects of current research methods of natural products, an LC-MS-DS that consisted of more than 600 natural products was constructed with HPLC-ESI-IT-MS/MS. The database searching results of LC-MS-DS were validated to be reliable, which indicated that LC-MS-DS could be successfully applied for the effective identification and target isolation of natural products. The study will provide an effective method for fast identification and efficient isolation of natural products.