郭, 颖, 胡玉芳, 程桂芳. 炎性刺激剂对小鼠腹腔巨噬细胞NF-κB的诱导J. 药学学报, 1999, 34(8): 586-589.
引用本文: 郭, 颖, 胡玉芳, 程桂芳. 炎性刺激剂对小鼠腹腔巨噬细胞NF-κB的诱导J. 药学学报, 1999, 34(8): 586-589.
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Guo Ying Hu Yufang, Cheng Guifang, . INDUCTION OF NF-κB IN MOUSE PERITONEAL MACROPHAGES BY INFLAMMATORY IRRITANTSJ. Acta Pharmaceutica Sinica, 1999, 34(8): 586-589.
Citation: Guo Ying Hu Yufang, Cheng Guifang, . INDUCTION OF NF-κB IN MOUSE PERITONEAL MACROPHAGES BY INFLAMMATORY IRRITANTSJ. Acta Pharmaceutica Sinica, 1999, 34(8): 586-589.
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炎性刺激剂对小鼠腹腔巨噬细胞NF-κB的诱导

INDUCTION OF NF-κB IN MOUSE PERITONEAL MACROPHAGES BY INFLAMMATORY IRRITANTS

    摘要
  • 摘要: 目的：建立炎性刺激剂诱导细胞核因子κB（Nuclear factor κB,　NF-κB）的模型，研究传统非甾体抗炎药阿斯匹林（aspirin）作用机理。 方法：用脂多糖（LPS）和佛波酯（PMA）刺激小鼠腹腔巨噬细胞，用电泳迁移率改变检测法（electrophoretic mobility shift assay,EMSA）检测。结果：LPS 1 μg.mL-1及3 μg.mL-1，PMA 2 ng.mL-1均能诱导细胞核内NF-κB的含量。阿斯匹林10-5 mol.L-1可以显著抑制LPS（1 μg.mL-1）和PMA（PMA 2 ng.mL-1）对细胞核内NF-κB的活化。结论：所建立的以LPS和PMA为刺激剂，诱导细胞核内NF-κB的模型，可用于非甾体抗炎药的抗炎机理的研究。

     

    Abstract: AIM: To establish the model of determining nuclear factor κB（NF-κB） which is stimulated by different inflammatory irritants in inducible peritoneal macrophages from mouse and investigate the inhibitory effect of aspirin on NF-κB activation using this model. METHODS: Lipopolysaccharide(LPS) and phorbol 12-myristate, 13-acetate(PMA) were used as irritants. Electrophoretic mobility shift assay (EMSA) was used as the determination method. RESULTS: LPS(μg.mL-1， 3 μg.mL-1) and PMA (1 ng.mL-1) was shown to increase the content of NF-κB in inducible peritoneal macrophages from the mouse. After the macrophages were treated with aspirin(10-7 mol.L-1,　10-6 mol.L-1, 10-5 mol.L-1),　the increase was inhibited. CONCLUSION: The model of determining NF-κB stimulated by LPS and PMA can be used in researching mechanism of non-steroidal anti-inflammatiory drugs.

     

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