多肽研究XX:丙型肝炎病毒(HCV)合成多肽的抗原性及线性抗体谱

STUDIES ON SYNTHETIC PEPTIDE XX:THE ANTIGENICITY AND LINEAR EPITOPE MAP OF SYNTHETIC PEPTIDE HEPATITIS C VIRUS

摘要: 依据蛋白质的亲水性、可亲性、柔韧性、抗原性、电荷分布及HPLC滞留系数等六种性质,使用“Goldkey”软件系统给出了HCV-BK各蛋白抗原决定簇预测曲线,共设计、合成15个多肽片段:P1(475～495),P3(449～468),P4(658～663),P5(645～663),P6(484～489),P7(475～489),P15(655～662),P16(230～237),P17(225～237),P18(1220～1240),P19(1694～1735),P24(1230～1240),P25(1482～1493),P26(384～389)和P27(2355～2389)。发现NS1区%和NS4区P19有很强的抗原性。用其检测PT-HC,阳性率分别为60%和63%。

Abstract: Hepatitis C virus(HCV),the major causative agent of post transfusiori non-A,non-B hepatitis(NANB),had been cloned and expressed³. According to the protein sequence ofHCV-BK and its epitope profiles which combined the hydrophilicity,accessibility,flexibility,antigenicityl charge distribution and HPLC reserve coefficient of protein using the“Goldkey”computer program,we designed and synthesized the following peptides:P1(475～495),P3(449～468),P4(658～663),P5(645～663),P6(484～489),P7(475～489),P15(655～662),P16(230～237),P17(225～237),P18(1220～1240),P19(1694～1735),P24(1230～1240),P25(1482～1493), P26(384～389),P27(2355～2389).The results of ELISA showed that P6(60% positiveresults)and P19(63% positive results)testing with PT-HC of Gu An,Hebei province were the majorantigens in NS1 and in NS4 region,respectively.