茶中儿茶素类和生物碱成分的HPLC分析

STUDIES ON ANALYSIS OF CATECHINSAND ALK ALOI DSINTEABYHPLC

摘要: 建立了以恒组成溶液为流动相的HPLC同时分析茶中5种儿茶素类(C,ECG,EC,EGC,EGCG)和3种生物碱(caf,Tp,Tb)的定量方法,色谱条件为:ODS(10μm)柱,以0.04mol·L^-1柠檬酸水溶液-N,N-二甲基甲酰胺-四氢呋喃(90:16:4)为流动相,UV检测器(278nm),并以二氯磺胺为内标。结果表明:8种成分分离良好,EGCG(7,2%～9.4%)和caf(4.4%～4.7%)分别为儿茶素类和生物碱中含量最高的成分。同时探讨了这些成分的提取方法和分离测定条件。

Abstract: By using HPLC, five catechins (catechin ,epicatechin ,epigallocatechin,epicatechingallate, epigallocatechin gallate )and three tea alkaloids(eaffeine,theobromine, theophylline )wereseparated and determined simultaneously. The chromatographic conditions were as follows: stainlesssteel column, ODS(Nucleosil,10μm),0. 04 mol·L^-1 citricacid solution, dimethyl formamide andtetrahydrofuran (96: 16: 4) as mobile phase,UV detector(at 278 nm). The method is simple,convcnient andsensitive .