李蓓佳 向诚 杨秀伟 吴立军 果德安 叶 敏. 应用高效液相色谱-质谱联用技术研究通脉颗粒的指纹图谱J. 药学学报, 2010,45(11): 1410-1414.
引用本文: 李蓓佳 向诚 杨秀伟 吴立军 果德安 叶 敏. 应用高效液相色谱-质谱联用技术研究通脉颗粒的指纹图谱J. 药学学报, 2010,45(11): 1410-1414.
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LI Bei-Jia, XIANG Cheng, YANG Xiu-Wei, TUN Li-Jun, GUAN De-An, XIE Min. Fingerprints of Tongmai Keli by HPLC-DAD-MSJ. 药学学报, 2010,45(11): 1410-1414.
Citation: LI Bei-Jia, XIANG Cheng, YANG Xiu-Wei, TUN Li-Jun, GUAN De-An, XIE Min. Fingerprints of Tongmai Keli by HPLC-DAD-MSJ. 药学学报, 2010,45(11): 1410-1414.
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应用高效液相色谱-质谱联用技术研究通脉颗粒的指纹图谱

Fingerprints of Tongmai Keli by HPLC-DAD-MS

    摘要
  • 摘要:

    为了比较全面并且快速地阐明中药复方通脉颗粒的化学组成, 本文建立了通脉颗粒的高效液相色谱 (HPLC-UV) 指纹图谱, 并利用液相-二极管阵列-质谱联用 (LC-DAD-MS) 技术, 从通脉颗粒中鉴定了22个化合物, 并归属了各化合物的单味药来源。结果显示通脉颗粒的主要成分包括染料木素8-C-葡萄糖苷、葛根素、大豆素8-C-芹糖 (1, 6)-O-葡萄糖苷、大豆苷以及丹酚酸B。又对10批来自不同生产厂家的样品进行HPLC分析, 发现各样品的化学组成相似, 但主要色谱峰的面积差异显著。该研究比较全面地阐明了通脉颗粒的化学组成, 为此中药复方制剂的质量控制奠定基础。

     

    Abstract:

    In order to clarify the chemical composition of Tongmai Keli, a HPLC fingerprint was established, and the 22 peaks were characterized by LC-DAD-MS.  The herbal sources of these peaks were assigned.  The results implied that genistin 8-C-glucoside, puerarin, daidzein 8-C-apiosyl (1, 6)-O-glucoside, daidzin, and  salvianolic acid B were the main constituents of in Tongmai Keli.  Ten batches of Tongmai Keli produced  by different pharmaceutical companies were analyzed.  Although different batches contained similar compounds, the contents of major compounds were significantly different.  The method established in this study could be used for the quality control of Tongmai Keli.

     

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