Abstract: AimTo investigate the molecular mechanism of protective effect of acidic oligose 971 on Alzheimer’s disease mouse model by using microarray. MethodsBalb/c mice were randomly divided into control group, β-AP_25-35 icv injected group and 971-treated group. The learning-memory ability of mice was tested by Morris water maze experiment. Total RNA of the cerebral cortex was extracted from the mice of each group. cDNA microarrays containing 1 176 genes were used to investigate the gene expression pattern of each group. Expressions of 5 genes were randomly selected for further confirmation by RT-PCR. ResultsIcv injection of β-AP_25-35 caused significant impairments in spatial and working memory performances of mice in Morris water maze and which were relieved by the treatment of 971. Up- and down- regulated genes were 19 and 12 in β-AP_25-35-injected group vs control group, respectively. Up- and down- regulated genes were 13 and 4, respectively, in 971-treated group vsβ-AP_25-35-injected group. RT-PCR results indicated that 5 genes showed identical results to that of the microarray. ConclusionThe protective effect of 971 on learning and memory ability of β-AP25-35-treated mouse may be related to the expression changes of genes involved in cell cycle, DNA repair, nerve growth, synaptic plasticity and immune response, etc.