Abstract: high speed countercurrent chromatography (HSCCC)based on the fundamental principle of liquid-liquid partition is a newly developed technique for isolation. It utilizes a particular combination of coil orientation and planetary motion to produce a unique hydrodynamic phenomenon in the unilateral phase distribution of two immiscible solvents in a coiled column. In recent years HSCCC has gained increasing popularity in the isolation of bioactive natural products. This paper reports its application in the prepartive separation of taxol (1), cephalomannine (2)and baccatin-Ⅲ (3) from the alcoholic extract of Taxus yunnanensis. These 3 ingredients are first obtained from this plant.