Abstract: The effects of phenylalanine, sucrose and mannitol on the cell growth and the production of taxol, baccatin III and 10-deacetylbaccatin III in the suspension cells of Taxus media were studied. The results showed that phenylalanine 1.0 mmol·L^-1 or 2.0 mmol·L^-1 initially added into the medium, and sucrose 73.0 mmol·L^-1 and mannitol 173.3 mmol·L^-1 added into the medium at the 28^th d of culture strongly promoted the cell growth and the formation of the three taxanes in the suspension cells. Compared with those of the control, the cell biomass of the treatments supplemented with phenylalanine and added with sucrose and mannitol at the 28^th d of culture increased by 0.6～0.8-fold, taxol yield by 9～10-fold, baccatin III yield by 2.5～3.0-fold, and 10-deacetylbaccatin III yield by 7-old. Addition of sucrose 73.0 mmol·L^-1 at the 28^th d of culture significantly promoted the cell growth, but showed little effect on the contents of the three taxanes in the suspension cultures.