那路新, 刘欣, 蒙卓明, 关注, 张礼和, 杨振军. 生物素光亲和分子的设计合成及其对2'-O-炔丙基鸟苷的标记J. 药学学报, 2015,50(1): 59-63.
引用本文: 那路新, 刘欣, 蒙卓明, 关注, 张礼和, 杨振军. 生物素光亲和分子的设计合成及其对2'-O-炔丙基鸟苷的标记J. 药学学报, 2015,50(1): 59-63.
shu
NA Lu-xin, LIU Xin, MENG Zhuo-ming, GUAN Zhu, ZHANG Li-he, YANG Zhen-jun. Design and synthesis of photoaffinity biotin labelled 2'-O-propargyl-guanosineJ. Acta Pharmaceutica Sinica, 2015,50(1): 59-63.
Citation: NA Lu-xin, LIU Xin, MENG Zhuo-ming, GUAN Zhu, ZHANG Li-he, YANG Zhen-jun. Design and synthesis of photoaffinity biotin labelled 2'-O-propargyl-guanosineJ. Acta Pharmaceutica Sinica, 2015,50(1): 59-63.
shu

生物素光亲和分子的设计合成及其对2'-O-炔丙基鸟苷的标记

Design and synthesis of photoaffinity biotin labelled 2'-O-propargyl-guanosine

    摘要
  • 摘要: 本文基于光亲和生物素探针分子在小分子配体靶标确证领域的应用, 设计并合成了带有叠氮基的光亲和生物素探针分子1, 证实其能够通过点击化学反应, 对 N2-乙酰基-2'-O-炔丙基鸟苷9进行标记。为进一步对环核苷酸信使分子及反义寡聚核苷酸和siRNA等进行光亲和生物素标记提供了方法。

     

    Abstract: Photoaffinity labeling is widely applied to demonstrate targets of small molecule ligands. In this paper, biotin photoaffinity labeled molecule with propargyl group 1 has been designed and synthesized, followed it's labeling of N2-acetyl-2'-O-propargyl guanosine 9 by "click chemistry". This technology presents delight development potential in labeling of second messenger cyclic nucleotide, antisense oligonucleotide or siRNA.

     

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