血卟啉衍生物—光辐射对L1210白血病细胞核酸生物合成的影响

THE EFFECTS OF HEMATOPORPHYRIN DERIVATIVES——PHOTOIRRADIATION ON DNA AND RNA SYNTHESES IN L1210 CELLS

摘要: 本文报道几种血卟啉衍生物—光辐射效应对L1210白血病细胞DNA及RNA生物合成有不同程度的抑制。对DNA生物合成抑制的IC₅₀,HA 308为2.1μg/ml,激光3号为2.5μg/ml,LF-019为3.6μg/ml,Photofrin Ⅱ>5μg/ml,对RNA生物合成抑制的IC₅₀ LF-019为3.7μg/ml,激光3号为5.7 μg/ml,HA 308为8.8 μg/ml,photofrin Ⅱ>10 μg/ml.其中激光3号对DNA聚合酶α的光动力学失活作用也较明显。

Abstract: The effects of hematoporphyrin derivatives (HPD) photoirradiation on DNA and RNA syntheses in L1210 cells were studied. The inhibition of ³H-TdR incorporation into DNA by HPD was significant, the IC₅₀ of HA 308, Jiguang 3, LF-019 and photofrin Ⅱ were 2.1, 2.5, 3.6 and >5 μ/ml, respectively. The synthesis of RNA (³H-UR incorporation) was also suppressed, the IC₅₀ for LF-019, Jiguang 3, HA 308, and Photofrin Ⅱ were 3.7, 5.7, 8.8 and >10 μg/ml, respectively. The photodynamic inactivation of DNA polymerase by hematoporphyrin derivative and light was noted.