Abstract: In addition to atisine and talatisamine, two new alkaloids, gymnaconitine (C), C₃₄H₄₇O₈N, and methylgymnaconitine (D), C₃₅H₄₉O₈N, have been isolated from Aconitum gymnandrum Maxim. The chemical structures of gymnacontine and methylgymnaconitine were established by UV, IR, ¹H NMR, ¹³C NMR and MS spectral data and chemical evidence.It should be pointed out that it is found for the first time in the research of Aconitum alkaloids that both gymnaconitine and methylgymnaconitine contain 3,4-O-dimethyl-caffeic ester.