时丽丽 强桂芬 高梅 张恒艾 陈柏年 于晓彦 玄昭红 王巧云 杜冠华. 匹诺塞林对脑线粒体呼吸功能的促进作用J. 药学学报, 2011,46(6): 642-649.
引用本文: 时丽丽 强桂芬 高梅 张恒艾 陈柏年 于晓彦 玄昭红 王巧云 杜冠华. 匹诺塞林对脑线粒体呼吸功能的促进作用J. 药学学报, 2011,46(6): 642-649.
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SHI Li-Li, JIANG Gui-Fen, GAO Mei, ZHANG Heng-Ai, CHEN Bai-Nian, XU Xiao-Pan, XUAN Zhao-Gong, WANG Qiao-Yun, DU Guan-Hua. Effect of pinocembrin on brain mitochondrial respiratory functionJ. 药学学报, 2011,46(6): 642-649.
Citation: SHI Li-Li, JIANG Gui-Fen, GAO Mei, ZHANG Heng-Ai, CHEN Bai-Nian, XU Xiao-Pan, XUAN Zhao-Gong, WANG Qiao-Yun, DU Guan-Hua. Effect of pinocembrin on brain mitochondrial respiratory functionJ. 药学学报, 2011,46(6): 642-649.
shu

匹诺塞林对脑线粒体呼吸功能的促进作用

Effect of pinocembrin on brain mitochondrial respiratory function

    摘要
  • 摘要:

    匹诺塞林是一种广泛存在于蜂胶和植物中的黄酮类天然化合物, 具有抗菌、抗炎、抗氧化、神经保护等多种药理作用。本实验利用大鼠全脑缺血模型 (4-vessel occlusion, 4-VO), 评价了匹诺塞林在体内对4-VO大鼠脑线粒体功能的保护作用。结果表明, 匹诺塞林能显著提高4-VO大鼠脑线粒体的ADP/O比值、V3以及RCI、OPR等参数, 保护线粒体结构完整性; 体外实验表明, 匹诺塞林能显著提高分离的脑线粒体的ADP/O、V3、RCI、OPR, 降低V4, 保护线粒体结构完整性; 同时, 匹诺塞林在体外条件下促进脑线粒体ATP的生成; 在细胞水平, 匹诺塞林能促进SH-SY5Y细胞的ATP生成。本研究发现, 匹诺塞林通过保护线粒体结构完整性, 降低NADH呼吸链的电子漏流, 提高氧化磷酸化效率, 促进线粒体呼吸功能, 提升线粒体ATP合成能力等起到脑缺血保护作用。

     

    Abstract:

    There are growing evidences that pinocembrin has better neuroprotective effect.  In the present study, the effect of pinocembrin on mitochondrial respiratory function was evaluated in global brain ischemia/ reperfusion (4-vessel occlusion, 4-VO) rats.  The results showed that pinocembrin improved the respiratory activity of 4-VO brain mitochondria, through increasing ADP/O, state 3 respiration state (V3), respiration control rate index (RCI) and oxidative phosphorylation rate (OPR).  And then, the effect of pinocembrin on brain mitochondria was verified in vitro.  The results showed that pinocembrin increased ADP/O, state 3 respiration state, respiration control rate index, oxidative phosphorylation rate in NADH/FADH2 dependent respiratory chain and decreased state 4 respiration state (V4) in NADH dependent respiratory chain.  Pinocembrin improved ATP content in brain mitochondria in vitro and in SH-SY5Y cells.

     

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