膝沟藻毒素Gonyautoxins的分离纯化膝沟藻毒素Gonyautoxins的分离纯化

缪宇平; 周宏农; 闻韧

膝沟藻毒素Gonyautoxins的分离纯化膝沟藻毒素Gonyautoxins的分离纯化

Isolation and purification of gonyautoxins from Alexandriun minutum Halim

摘要

摘要: 目的从微小亚历山大藻(Alexandriun minutum Halim)Amtk 2中分离纯化膝沟藻毒素gonyautoxins。方法用凝胶色谱、离子交换色谱等方法从人工培养的微小亚历山大藻Amtk 2酸酒精萃取物中分离纯化膝沟藻毒素。结果从100 L Amtk 2培养液中获得(6.74±0.31)×109个藻细胞,其酸酒精萃取物经凝胶色谱分离首次在国内得到膝沟藻毒素GTX-4,GTX-1,GTX-3和GTX-2混合物(29.59±0.28) mg。从此混合物中取出4.06 mg经两次离子交换色谱纯化得到纯GTX-4(0.40±0.002) mg, GTX-1(5.95±0.03)×10^-2 mg,GTX-3(6.92±0.05)×10^-4 mg和GTX-2(0.11±0.005) mg。结论人工培养的微小亚历山大藻Amtk 2酸酒精萃取物通过凝胶色谱和两次离子交换色谱分离能够得到纯的膝沟藻毒素GTX-4,GTX-1,GTX-3和GTX-2。

Abstract: AimTo isolate and purify gonyautoxins from Alexandriun minutum Halim Amtk2 strain. MethodsThe ethanol extracts of culture Alexandriun minutum Halim Amtk2 were isolated by means of gel filtration chromatography, the toxin fraction obtained was then purified by ion exchange chromatography. Results From 100 liter of cultivation liquid of Alexandriun minutum Halim Amtk2 (6.74±0.31)×10⁹cells were obtained. The ethanol extracts of Alexandriun minutum Halim purified by gel filtration chromatography obtained gonyautoxins mixture 29.59 mg. 4.06 mg of the mixture was further purified by two steps of ion exchange chromatography, and obtained pure GTX-4 (0.40±0.002) mg, GTX-1 (5.95±0.03)×10^-2 mg, GTX-3 (6.92±0.05)×10^-4 mg and GTX-2 (0.11±0.005) mg. ConclusionPure gonyautoxins can be obtained by means of gel filtration chromatography and ion exchange chromatography from ethanol extracts of cultured Alexandriun minutum Halim Amtk2 strain.

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