张大为, 赵明明, 陈 娟, 李 超, 郭顺星. 西藏药用植物螃蟹甲可培养内生真菌的分离、鉴定及抗HIV-1整合酶链转移活性研究J. 药学学报, 2013,48(5): 780-789.
引用本文: 张大为, 赵明明, 陈 娟, 李 超, 郭顺星. 西藏药用植物螃蟹甲可培养内生真菌的分离、鉴定及抗HIV-1整合酶链转移活性研究J. 药学学报, 2013,48(5): 780-789.
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ZHANG Da-wei, ZHAO Ming-ming, CHEN Juan, LI Chao, GUO Shun-xing. Isolation, idetification and anti-HIV-1 integrase activity of culturable endophytic fungi from Tibetan medicinal plant Phlomis younghusbandii MukerjeeJ. 药学学报, 2013,48(5): 780-789.
Citation: ZHANG Da-wei, ZHAO Ming-ming, CHEN Juan, LI Chao, GUO Shun-xing. Isolation, idetification and anti-HIV-1 integrase activity of culturable endophytic fungi from Tibetan medicinal plant Phlomis younghusbandii MukerjeeJ. 药学学报, 2013,48(5): 780-789.
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西藏药用植物螃蟹甲可培养内生真菌的分离、鉴定及抗HIV-1整合酶链转移活性研究

Isolation, idetification and anti-HIV-1 integrase activity of culturable endophytic fungi from Tibetan medicinal plant Phlomis younghusbandii Mukerjee

    摘要
  • 摘要:

    从西藏药用植物螃蟹甲 (Phlomis younghusbandii Mukerjee) 的叶和茎中分离得到52株内生真菌。经分子鉴定 (基于ITS序列和28S序列), 内生真菌分属于Phoma、Chaetosphaeronema、Fusarium、Leptosphaeria等12属, 其中拟茎点霉属 (Phoma) 为其优势菌群。使用原核表达的可溶型一型人类免疫缺陷病毒 (human immunodeficiency virus type 1, HIV-1) 的整合酶 (integrase, IN), 对52株内生真菌的发酵产物进行体外抗HIV-1整合酶链转移反应活性试验, 结果发现, 来自Chaetosphaeronema属真菌的7个样品对整合酶链转移反应表现出强烈的抑制作用, 其对应的IC50值分别为6.60、5.20、2.86、7.86、4.47、4.56和3.23 µg?mL−1。该研究表明, 螃蟹甲内生真菌在发现抗HIV-1整合酶活性物质方面有进一步的开发价值。

     

    Abstract:

    A total of 52 endophytic fungi were isolated from roots and stems of Tibetan medicinal plant Phlomis younghusbandii Mukerjee.  These fungal isolates were molecularly identified based on ITS sequnces and 28S sequences distributed to 12 genera, including Phoma, Chaetosphaeronema, Fusarium and Leptosphaeria, etc.  Among them, the dominant genus was Phoma.  Extracts of all strains were evaluated for anti-HIV-1 integrase activity by using soluable integrase expressed in E. coli BL21 (DE3).  The results showed that seven samples from five fungal endophytes PHY-24, PHY-38, PHY-40, PHY-51, PHY-53, which belonged to genus Chaetosphaeronema, inhibited strand transfer reaction catalyzed by HIV-1 integrase with IC50 values of 6.60, 5.20, 2.86, 7.86, 4.47, 4.56 and 3.23 µg?mL−1 respectively.  In conclusion, the endophytic fungi of Phlomis younghusbandii Mukerjee are valuable for further screening anti-HIV-1 integrase agents.

     

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