丁岗, 陆蕴如, 冀春茹, 刘延泽. 诃子及其易混淆品中二种丹宁成分的高效毛细管电泳分析J. 药学学报, 2001, 36(4): 292-295.
引用本文: 丁岗, 陆蕴如, 冀春茹, 刘延泽. 诃子及其易混淆品中二种丹宁成分的高效毛细管电泳分析J. 药学学报, 2001, 36(4): 292-295.
DING Gang, LU Yun-ru, JI Chun-ru, LIU Yan-ze. ANALYSIS OF TANNINS IN FRUCTUS CHEBULAE AND ITS CONFUSION VARIETIES BY HPCEJ. Acta Pharmaceutica Sinica, 2001, 36(4): 292-295.
Citation: DING Gang, LU Yun-ru, JI Chun-ru, LIU Yan-ze. ANALYSIS OF TANNINS IN FRUCTUS CHEBULAE AND ITS CONFUSION VARIETIES BY HPCEJ. Acta Pharmaceutica Sinica, 2001, 36(4): 292-295.

诃子及其易混淆品中二种丹宁成分的高效毛细管电泳分析

ANALYSIS OF TANNINS IN FRUCTUS CHEBULAE AND ITS CONFUSION VARIETIES BY HPCE

  • 摘要: 目的对不同来源的诃子及其易混淆品中的可水解丹宁:诃子酸(chebulinicacid,I)、诃黎勒酸(chebulagicacid,II)进行定量分析。方法未涂层石英毛细管50μm×81.5cm,有效长度61.5cm,混合缓冲溶液为20mmol·L-1Na2HPO4+60mmol·L-1硼酸,运行电压24kV,真空进样5s,检测波长280nm。结果I和II的线性范围分别为0.0842-0.842和0.0940-0.940mg·mL-1,相关系数分别为0.9966和0.9957,加样回收率分别为95.57%(RSD=4.0%)和94.99%(RSD=4.4%),精密度试验RSD分别为2.2%和1.7%,重现性试验RSD分别为5.4%和4.0%。I和II的含量与诃子的品种、性状密切相关。结论I和II可作为评价诃子药材质量的指标性成分,HPCE法是评价诃子药材质量的有效方法。

     

    Abstract: AIM To analyze the hydrolyzable tannins chebulinic acid (I) and chebulagic acid(II) in Fructus Chebulae and its confusion varieties by using high performance capillary electrophoresis (HPCE) method. METHODS Using a capillary (375 μm OD×50 μm ID; 81.5 cm×61.5 cm) and a power supply set at 24 kV, with phosphate-borate buffer containing 20 mmol·L-1 Na2HPO4-60 mmol·L-1 boric acid and a UV detector at 280 nm, sample solution was loaded in decompression mode at the positive end of the capillary, the loading time was 5 s. RESULTS The linear ranges of I and II were 0.0842-0.842 and 0.0940-0.940 mg·mL-1 respectively, the correlation coefficient were 0.9966 and 0.9957, the average recoveries were 95.6% (RSD=4.0%, n=5) and 95.0% (RSD=4.4%, n=5), the RSDs (n=5) of measurement precision test were 2.2% and 1.7%, the RSDs (n=6) of reproduction test were 5.4% and 4.0% respectively. The contents of I and II were obviously interrelated with the variety and characteristics of Fructus Chebulae, the contents of I and II in the confusion varieties of Fructus Chebulae were very low. CONCLUSION It is suitable to use I and II as the criterion in quality evaluation of Fructus Chebulae, and the HPCE method is effective for quality evaluation of the crude Fructus Chebulae.

     

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