Syringin is one of the main bioactive ingredients in Saussurea involucrata.  In this study, various chromatographic techniques were employed to isolate and purify syringin in the polar extraction of cell suspension cultures of S. involucrata.  The structure of syringin was characterized by the analysis of spectroscopic data.  A quantitative analytical method for the content of syringin in cultures of S. involucrata was established with RP-HPLC.  The method is convenient, accurate and reliable.  All this results provided a basis for further  studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive   compound syringin.