微渗析结合RP-HPLC研究盐酸平阳霉素在家兔血中的药代动力学

Pharmacokinetics of pingyangmycin hydrochloride in rabbits determined by microdialysis coupled with RP-HPLC

摘要: 采用微渗析技术研究盐酸平阳霉素在体药代动力学，用Supelco RP-amide C₁₆柱进行样品的HPLC分析，盐酸平阳霉素的血药浓度用3P87程序处理。结果表明，家兔静注盐酸平阳霉素后，渗析液中的药物浓度在1.04～66.56 μg·mL^-1时，浓度与峰面积有良好的线性关系(R²=0.999 4)。微渗析探针的在体回收率为(42.8±3.4)%(n =6)。所测血药浓度经3P87程序拟合后，药-时曲线符合二室模型，T_1/2α及T_1/2β分别为14.9及60.3 min。因此，该方法可用于盐酸平阳霉素药代动力学研究。

Abstract: Microdialysis coupled with RP-HPLC was used to study the blood pharmacokinetics of pingyangmycin hydrochloride in rabbits. Supelco RP-amide C₁₆ column was adopted for the analysis of pingyangmycin hydrochloride. The data was analyzed with 3P87 program. The calibration curve was linear in the concentration range from 1.04 to 66.56 μg·mL^-1 (R²=0.999 4)．The in vivo recovery of microdialysis probe was (42.8±3.4)% (n=4). The concentration-time curve of pingyangmycin hydrochloride was fitted to two-compartment model. T_1/2αand T_1/2β were 14.9 and 60.3 min, respectively. The method is proved to be accurate，simple and suitable for the pharmacokinetics study of pingyangmycin hydrochloride in rabbits.