Abstract: A study on the HPLC method for the determination of ginsenosides (Rb₁, Rb₂, Rc, Rd, Re, Rg) in Panax ginseng with the refractometer detection within 15 min has been proposed. The effect of the composition of the mobile phase on separation was examined and the extraction and purification methods were improved.Macerate 1.0 g of pulverized sample with 25 ml of water saturated n-butanol overnight and then extract by ultrasonic method for 10 min. After centrifugation, take exactly 5 ml of the supernatant liquid and evaporate to dryness. Dissolve the residue in water and purify with macroporous resin, take the 70% ethanol elute to dryness again and redissolve the residue in 1 ml of mobile phase. Inject 10 μl of the sample solution and the standard solution onto a—NH₂ column (15 cm×4.6 mm, id) respectively. Run the chromatogram with methanol—acetonitrile—glycol—ammonium acetate (0.14 mol/L) (30:70:5:10.6) as mobile phase and calculate the results from the peak area.The calibration curves of all six saponins showed a favorable straight line relationship over the concentration range of 4～24 μg. Analytical results obtained by both HPLC and TLCD methods are in agreement.This method is rapid, reproducible and has been applied" to the comparison ofsaponin contents in various samples.