山莨菪碱(654-2)异构体的高效液相色谱法分离

SEPARATION OF EPIMERS OF ANISODAMINE (654-2) WITH HPLC USING CHIRAL ADDITIVES IN THE MOBILE PHASE

摘要: 本文采用手性流动相用高效液相色谱法对山良菪碱的合成品直接进行分离。所使用的色谱柱为ODS C₁₈柱,手性流动相中的手性物质为d-樟脑磺酸或L-(+)-酒石酸二正丁酯。当在流动相中使用d-樟脑磺酸或将d-樟脑磺酸与L-(+)-酒石酸二正丁酯合用时,样品中的一对差向异构体可得到基线分离,而在流动相中加入β-环糊精对山良菪碱的差向异构体分离没有改善作用。

Abstract: The separation of epimers of anisodamine were studied with HPLC using a reversed phase ODS C₁₈ column. Several chiral additives were tried in the mobile phases.d-Camphorsulfonic acid and L- (+)-dibutyl tartrate showed good resolution ability for the separation of the isomers, a pair of epimers of anisodamine were well separated; while β-cyclodextrin appeared to have no selectivity for the epimers.