潘国宇, 王广基, 孙建国, 黄志江, 赵小辰, 顾轶, 刘晓东. 小檗碱对葡萄糖吸收的抑制作用J. 药学学报, 2003, 38(12): 911-914.
引用本文: 潘国宇, 王广基, 孙建国, 黄志江, 赵小辰, 顾轶, 刘晓东. 小檗碱对葡萄糖吸收的抑制作用J. 药学学报, 2003, 38(12): 911-914.
shu
PAN Guo-yu, WANG Guang-ji, SUN Jian-guo, HUANG Zhi-jiang, ZHAO Xiao-chen, GU Yi, LIU Xiao-dong. Inhibitory action of berberine on glucose absorptionJ. Acta Pharmaceutica Sinica, 2003, 38(12): 911-914.
Citation: PAN Guo-yu, WANG Guang-ji, SUN Jian-guo, HUANG Zhi-jiang, ZHAO Xiao-chen, GU Yi, LIU Xiao-dong. Inhibitory action of berberine on glucose absorptionJ. Acta Pharmaceutica Sinica, 2003, 38(12): 911-914.
shu

小檗碱对葡萄糖吸收的抑制作用

Inhibitory action of berberine on glucose absorption

    摘要
  • 摘要: 目的研究小檗碱的吸收特性和对肠道葡萄糖吸收的影响,探索小檗碱抗糖尿病作用机制。方法采用在体肠灌流模型观察小檗碱的吸收特性;用Caco-2细胞模型研究小檗碱对肠上皮细胞二糖酶(麦芽糖酶)活力的影响和对葡萄糖转运蛋白的作用。结果小檗碱在肠道内几乎不吸收(2.5 h吸收量小于5%),但能有效抑制小肠上皮细胞上二糖酶活力,其抑制蔗糖酶的ID50为1.830 mg·L-1;对麦芽糖酶也有抑制作用,但无明显的剂量关系。小檗碱对于葡萄糖在Caco-2细胞上的摄取也有一定抑制作用。结论小檗碱抗糖尿病作用可能主要通过抑制蔗糖酶、麦芽糖酶等二糖酶活力,作为一种α-葡糖苷酶抑制剂发挥其抗糖尿病作用。

     

    Abstract: AimTo study the absorption characteristics of berberine and its influence on glucose absorption. MethodsRat recirculating perfusion model was used to study berberine absorption characteristics and Caco-2 cell model was used to explore the influence of berberine on disaccharidase, using HPLC to assay the appearance of glucose to indicate enzyme activities. ResultsBerberine was found to be hardly absorbed in the intestine (less than 5% in 2.5 h). However, sucrase and maltase activities were found to be inhibited by berberine, its ID50 to sucrase is 1.830 mg·L-1, and showed no dose dependent influence on maltase activity. Berberine also showed influence on glucose absorption. However, this effect is not significant. Conclusion Berberine may act as an alpha-glucosidase inhibitor, which is its main mechanism in diabetes treatment.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回