邓友平, 林晨, 张雪艳, 陈德权, 肖培根, 吴旻. 三氧化二砷(As2O3)诱导人胃腺癌SGC7901细胞程序化死亡并降低c-myc基因的表达J. 药学学报, 1999, 34(5): 333-337.
引用本文: 邓友平, 林晨, 张雪艳, 陈德权, 肖培根, 吴旻. 三氧化二砷(As2O3)诱导人胃腺癌SGC7901细胞程序化死亡并降低c-myc基因的表达J. 药学学报, 1999, 34(5): 333-337.
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Deng Youping, Lin Chen Zhang Xueyan, Chen Dequan, Xiao Peigen , Wu Min, . ARSENIC TRIOXIDE INDUCES PROGRAMMED CELL DEATH OF HUMAN GASTRIC ADENOCARCINOMA SGC7901 CELLS AND DECREASES C-MYC GENE EXPRESSIONJ. Acta Pharmaceutica Sinica, 1999, 34(5): 333-337.
Citation: Deng Youping, Lin Chen Zhang Xueyan, Chen Dequan, Xiao Peigen , Wu Min, . ARSENIC TRIOXIDE INDUCES PROGRAMMED CELL DEATH OF HUMAN GASTRIC ADENOCARCINOMA SGC7901 CELLS AND DECREASES C-MYC GENE EXPRESSIONJ. Acta Pharmaceutica Sinica, 1999, 34(5): 333-337.
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三氧化二砷(As2O3)诱导人胃腺癌SGC7901细胞程序化死亡并降低c-myc基因的表达

ARSENIC TRIOXIDE INDUCES PROGRAMMED CELL DEATH OF HUMAN GASTRIC ADENOCARCINOMA SGC7901 CELLS AND DECREASES C-MYC GENE EXPRESSION

    摘要
  • 摘要: 目的：探讨As2O3对胃腺癌SGC7901细胞系的生物学效应及机制。方法：通过MTT还原法检测As2O3对该细胞系存活率的影响，从光学显微镜形态观察，流式细胞仪分析，DNA凝胶电泳，细胞凋亡原位检测(TUNEL)进行细胞凋亡的检测。半定量RT-PCR检测基因表达。结果：As2O3处理SGC7901细胞后,细胞的存活率明显降低，光学显微镜下可见到明显的凋亡细胞,流式细胞仪测定细胞周期的G1期前有亚2倍体的凋亡峰，DNA凝胶电泳显示出典型的凋亡特征:　DNA有规律断裂形成的梯状图谱,细胞凋亡原位检测发现DNA的断裂,并降低细胞c-myc基因的表达。结论：As2O3能诱导人胃腺癌SGC7901细胞程序化死亡并可能通过降低c-myc基因的表达。

     

    Abstract: AIM: To study the biological effect of As2O3 on human gastric adenocarcinoma SGC7901 cells and its mechanisms. METHODS: MTT reduction assay, morphology investigation, flow cytometry analysis, DNA gel electrophoresis and In situ cell death detection (TUNEL), semiquantitive RT-PCR were adopted. RESULTS: As2O3 inhibited the survival of SGC7901 cell line. The cells treated with As2O3 showed a typical apoptotic morphology and hypodiploid peak before G1 phase. DNA of the treated SGC7901 cells appeared a ladder pattern characteristic of apoptosis. TUNEL detection analysis also revealed DNA fragmentation. Moreover, As2O3 decreased the c-myc gene expression. CONCLUSION: As2O3 can induce programmed death of SGC7901 cells mainly via down regulation of c-myc gene expression.

     

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