蜂毒多肽空间稳定免疫脂质体的制备及体外对肿瘤细胞的选择性

胡海洋; 陈大为; 刘彦仿; 乔明曦; 赵秀丽

蜂毒多肽空间稳定免疫脂质体的制备及体外对肿瘤细胞的选择性

Preparation and in vitro tumor cells selectivity of sterically stabilized immunoliposomal peptides in bee venom

摘要

摘要: 蜂毒多肽在肿瘤治疗中的应用引起研究者的极大兴趣。本研究使用大豆磷脂、胆固醇、羧酸化PEG-胆固醇制备了蜂毒多肽空间稳定脂质体，并将二硫键稳定抗人肝癌单链抗体联结PEG-胆固醇末端。使用酶联免疫法考察了蜂毒多肽空间稳定免疫脂质体的活性。蜂毒多肽空间稳定免疫脂质体有较高的肿瘤细胞选择性。体外实验证明，其对SMMC-7721细胞的杀伤能力远强于蜂毒多肽空间脂质体，而对Hela细胞的杀伤能力与蜂毒多肽空间脂质体无区别。蜂毒多肽空间稳定免疫脂质体对肿瘤细胞的选择性，可使其成为一种有效的靶向制剂。

Abstract: Recently the use of peptides in bee venom (PBV) for cancer therapy has attracted considerable attention. In this study, the sterically stabilized liposomal PBV (PBV-SL) was prepared using soybean phosphatidylcholine, cholesterol, and cholesterol-PEG-COOH. The humanized anti-hepatoma disulfide-stabilized Fv (hdscFv25) was coupled to sterically stabilized liposomes using the N-hydroxysuccinimide ester method. The hdscFv25-immunoliposomes (SIL［hdscFv25］) were immunoreactive as determined by ELISA assay. SIL［hdscFv25］ showed higher tumor cells selectivity. PBV-SIL［hdscFv25］ can kill SMMC-7721 cells in vitro with higher efficiency than non-targeted liposomes. Whereas cytotoxicties were compared for Hela cells, no significant differences was observed between PBV-SIL［hdscFv25］ and PBV-SL. Sterically stabilized immunoliposomal peptides in bee venom could be one drug targeting delivery system.

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