Abstract: Aim To develop a method for analysis of triterpene constituents in Ganoderma lucidum by HPLC. Methods Ganosporeric acid A (1), lucidenic acid A (2), ganoderic acid B (3) and ganoderic acid C (4) were used as comparison substances. Chromatographic column was a RP-C₁₈; the mobile phase was acetonitrile-water (37∶63); the flow rate was 1.0 mL·min^-1 and the detecting wavelength was 254 nm. Results A linear range was obtained from 0.2 μg to 1.0 μg with a good linear correlation. The recovery of (1), (2), (3) and (4) were 100.9%, 101.2%, 101.3% and 101.7%, respectively. Conclusion This method was developed for the analysis of triterpenes by HPLC for the first time. The method is rapid, accurate and suitable for the analysis of the triterpene constituents in fruit bodies, spores, mycelium from Ganoderma lucidum.