Abstract: To investigate whether lithium carbonate, propranolol or chloroquine aggravate psoriasis through influencing cytokines of the psoriatic cytokine network, HaCaT keratinocytes were stimulated with TNF-α after treatment with these drugs. Protein secretion of a set of multiple different cytokines and growth factors in culture supernatants were measured by using a cytokine antibody array technology. Expression of IL-8 and IL-6 mRNA was determined by real-time PCR. In culture supernatants of TNF-α-stimulated HaCaT cells, production of IL-6 and TNF-α could be enhanced by lithium carbonate; production of IL-6 and a panel of cytokines and growth factors could be enhanced by propranolol hydrochloride; and IL-6 was up-regulated by chloroquine diphosphate as well. Real-time PCR analysis showed a significantly dose-dependent increase of IL-8 and IL-6 mRNA expression in HaCaT cells stimulated with TNF-α as compared to cells without TNF-α-stimulation, the mRNA expression of IL-8 was higher than that of IL-6 with the same concentration of TNF-α (P<0.01). Compared with HaCaT cells cultured with medium alone, propranolol hydrochloride at the concentration of 1×10^-6 mol·L^-1 could stimulate HaCaT cells to express higher level of IL-6 mRNA (P<0.05). The drugs investigated show a modulatory effect on certain cytokines and growth factors which are able to modulate inflammatory type of immune reaction present in psoriatic lesions.