Abstract: The mechanism of enhancement of Bleomycin A₅ antitumor activity by verapamil was explored by flow cytometry and tracing the radiolabelled bleomycin A₅ in vivo. Verapamil was found to increase the G₂ blocking effect of bleomycin A₅ prominently in mouse S-180 and human HEP-2 cell lines. The distribution of ⁵⁷Co-bleomycin A₅ in mice bearing S-180 sarcoma was changed by verapamil and accumulation of the drug in tumor was increased. In contrast, the labelled drug in the lung was decreased. It seems that the effects of verapamil in enhancing the antitumor activity of bleomycin A₅ are to increase the accumulation of the drug in tumor cells and enhance the G₂ blocking effect of the drug in cell cycle.