孙国祥, 刘晓玲, 邓湘昱, 孙毓庆, 毕开顺. 色谱指纹图谱指数F和相对指数Fr的研究J. 药学学报, 2004, 39(11): 921-924.
引用本文: 孙国祥, 刘晓玲, 邓湘昱, 孙毓庆, 毕开顺. 色谱指纹图谱指数F和相对指数Fr的研究J. 药学学报, 2004, 39(11): 921-924.
SUN Guo-xiang, LIU Xiao-ling, DENG Xiang-yu, SUN Yu-qing, BI Kai-shun. The index F and relative index Fr of chromatographic fingerprintsJ. Acta Pharmaceutica Sinica, 2004, 39(11): 921-924.
Citation: SUN Guo-xiang, LIU Xiao-ling, DENG Xiang-yu, SUN Yu-qing, BI Kai-shun. The index F and relative index Fr of chromatographic fingerprintsJ. Acta Pharmaceutica Sinica, 2004, 39(11): 921-924.

色谱指纹图谱指数F和相对指数Fr的研究

The index F and relative index Fr of chromatographic fingerprints

  • 摘要: 目的提出色谱指纹图谱指数(F)和相对指数(Fr)概念,用FFr揭示中药色谱指纹图谱信息的丰富程度和分离情况,反映指纹信号强度的大小和均化程度。方法用HPLC和HPCE指纹图谱实验结果进行应用研究。结果评价了射干抗病毒注射液及其各单味药材的指纹图谱结果,并对文献中丹参HPLC指纹图谱进行了评价。当评价中药毛细管电泳指纹图谱时,其F无显著差别,但Fr是HPLC法的1000倍之上。结论FFr可客观、全面、简便地用于评价色谱指纹图谱。

     

    Abstract: AimThe index F and relative index Fr of chromatographic fingerprints were firstly proposed to indicate how bountiful was the information in traditional Chinese medicines chromatographic fingerprints,how better was the separation effect,how high was the peak signal and how equal were the peak areas. MethodsThe index F and relative index Fr of chromatographic fingerprints were firstly applied to evaluate the chromatographic fingerprints results of traditional Chinese medicines determined by the reversed-phase high performance liquid chromatographic method and high performance capillary electrophoresis. ResultsThe Shegan Kangbingdu injection and all its traditional Chinese medicines ingredients had been evaluated by F and Fr, so did for the HPLC fingerprints of Radix salviae miltiorrhizae reported in literature. As the same time, the F and Fr of the capillary electrophoresis fingerprints of Folium isatidis, Rhizoma belamcandae and compound liquorice tablets were successfully determined. As far as F was concerned, there was no evident difference between HPLC and capillary electrophoresis (CE), but the Fr values came from CE was usually a thousand times more than that from HPLC. ConclusionThe F and Fr can be applied to evaluate objectively, simply and thoroughly the chromatographic fingerprints.

     

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